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Proceedings Paper

Microfluidic device for capture and isolation of single cells
Author(s): Alexander P. Hsiao; Kristopher D. Barbee; Xiaohua Huang
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Paper Abstract

We describe a microfluidic device capable of trapping, isolating, and lysing individual cells in parallel using dielectrophoretic forces and a system of PDMS channels and valves. The device consists of a glass substrate patterned with electrodes and two PDMS layers comprising of the microfluidic channels and valve control channels. Individual cells are captured by positive dielectrophoresis using the microfabricated electrode pairs. The cells are then isolated into nanoliter compartments using pneumatically actuated PDMS valves. Following isolation, the cells are lysed open by applying an electric field using the same electrode pairs. With the ability to capture and compartmentalize single cells our device may be combined with analytical methods for in situ molecular analysis of cellular components from single cells in a highly parallel manner.

Paper Details

Date Published: 24 August 2010
PDF: 9 pages
Proc. SPIE 7759, Biosensing III, 77590W (24 August 2010); doi: 10.1117/12.861563
Show Author Affiliations
Alexander P. Hsiao, Univ. of California, San Diego (United States)
Kristopher D. Barbee, Univ. of California, San Diego (United States)
Xiaohua Huang, Univ. of California, San Diego (United States)

Published in SPIE Proceedings Vol. 7759:
Biosensing III
Hooman Mohseni; Manijeh Razeghi, Editor(s)

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