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Proceedings Paper

Green fluorescent nanodiamond conjugates and their possible applications for biosensing
Author(s): Joerg Opitz; Msau Mkandawire; Mathias Sorge; Norman Rose; Martin Rudolph; Peter Krueger; Inga Hannstein; Victoria A. Lapina; Dietmar Appelhans; Wolfgang Pompe; Juergen Schreiber; Gerhard Roedel
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Paper Abstract

Various nanoparticles play a prominent role in modern biosciences and medicine. Especially fluorescent cellular biomarkers are a prospective material for diagnostics and therapy. Nevertheless, most of the available biomarkers have some drawbacks due to either physical and optical or cytotoxic properties. Here we investigated the potential of green fluorescent nanodiamonds as extra- and intracellular biomarkers for living cells. We characterized the structure of the used detonation synthesized nanodiamonds (DND) by X-ray diffraction (XRD) and the optical properties by fluorescence and infrared spectroscopy. For the extracellular attachment the nanodiamonds were functionalized by attaching antibodies that target extracellular structures such as membrane. Transfections were mediated by dendrimers, cationic liposomes and protamine sulfate. Using fluorescence microscopy, we confirmed successful extracellular binding on and transfection of the nanodiamonds into prostate cancer cells. Furthermore, nanodiamonds can be targeted selectively to intracellular structures. Therefore, nanodiamonds are a promising tool for biosensing.

Paper Details

Date Published: 24 August 2010
PDF: 8 pages
Proc. SPIE 7759, Biosensing III, 775914 (24 August 2010); doi: 10.1117/12.860526
Show Author Affiliations
Joerg Opitz, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany)
Msau Mkandawire, Technische Univ. Dresden (Germany)
Mathias Sorge, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany)
Norman Rose, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany)
Martin Rudolph, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany)
Peter Krueger, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany)
Inga Hannstein, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany)
Victoria A. Lapina, B.I. Stepanov Institute of Physics (Belarus)
Dietmar Appelhans, Leibniz-Institut für Polymerforschung Dresdene e.V. (Germany)
Wolfgang Pompe, Technische Univ. Dresden (Germany)
Juergen Schreiber, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany)
Gerhard Roedel, Technische Univ. Dresden (Germany)

Published in SPIE Proceedings Vol. 7759:
Biosensing III
Hooman Mohseni; Manijeh Razeghi, Editor(s)

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