
Proceedings Paper
Probing the effect of elevated cholesterol on the mechanical properties of membrane-cytoskeleton by optical tweezersFormat | Member Price | Non-Member Price |
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Paper Abstract
The composition of the cell membrane and the surrounding physiological factors determine the nature and dynamics of
membrane-cytoskeleton coupling. Mechanical strength of a cell is mainly derived from such coupling. In this article, we
investigate the effect of extra cellular cholesterol on the membrane-cytoskelaton connectivity of single cell endothelium
and consequent remodeling of its mechanical properties. Using optical tweezers as a force probe, we have measured
membrane stiffness (km), membrane microviscosity (ηeff ) and the two-dimensional shear modulus (G′(f)) as a function of
extracellular cholesterol in the range of 0.1mM to 6mM. We find that membrane stiffness and shear modulus are
dependent on cholesterol-induced membrane-cytoskeletal organization. Further, by disrupting the membranecytoskeletal
connectivity with Cytochalasin D, an actin delpolymerizing molecule, we recover pure membrane behaviour
devoid of any cytoskeleton attachment. However, behaviour of ηeff was found to be unaffected by disruption of
membrane-cytoskeleton organization. We infer that cholesterol is playing a distinct role in modulating membrane
organization and membrane-cytoskeleton connectivity independently. We further discuss implications of our approach
in characterizing cellular mechanics.
Paper Details
Date Published: 2 September 2010
PDF: 13 pages
Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77621K (2 September 2010); doi: 10.1117/12.860464
Published in SPIE Proceedings Vol. 7762:
Optical Trapping and Optical Micromanipulation VII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)
PDF: 13 pages
Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77621K (2 September 2010); doi: 10.1117/12.860464
Show Author Affiliations
Arun S. Rajkumar, Anna Univ. (India)
Ajit Muley, Anna Univ. (India)
Ajit Muley, Anna Univ. (India)
Published in SPIE Proceedings Vol. 7762:
Optical Trapping and Optical Micromanipulation VII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)
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