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Proceedings Paper

Measurement of macrophage adhesion using optical tweezers with backward-scattered detection
Author(s): Sung-Yang Wei; Yi-Jr Su; Po-Chen Shih; Shih-Mo Yang; Long Hsu
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Paper Abstract

Macrophages are members of the leukocyte family. Tissue damage causes inflammation and release of vasoactive and chemotactic factors, which trigger a local increase in blood flow and capillary permeability. Then, leukocytes accumulate quickly to the infection site. The leukocyte extravasation process takes place according to a sequence of events that involve tethering, activation by a chemoattractant stimulus, adhesion by integrin binding, and migrating to the infection site. The leukocyte extravasation process reveals that adhesion is an important part of the immune system. Optical tweezers have become a useful tool with broad applications in biology and physics. In force measurement, the trapped bead as a probe usually uses a polystyrene bead of 1 μm diameter to measure adhesive force between the trapped beads and cell by optical tweezers. In this paper, using the ray-optics model calculated trapping stiffness and defined the linear displacement ranges. By the theoretical values of stiffness and linear displacement ranges, this study attempted to obtain a proper trapped particle size in measuring adhesive force. Finally, this work investigates real-time adhesion force measurements between human macrophages and trapped beads coated with lipopolysaccharides using optical tweezers with backscattered detection.

Paper Details

Date Published: 27 August 2010
PDF: 7 pages
Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77622M (27 August 2010); doi: 10.1117/12.860357
Show Author Affiliations
Sung-Yang Wei, National Chiao Tung Univ. (Taiwan)
Yi-Jr Su, National Chiao Tung Univ. (Taiwan)
Po-Chen Shih, National Chiao Tung Univ. (Taiwan)
Shih-Mo Yang, National Chiao Tung Univ. (Taiwan)
Long Hsu, National Chiao Tung Univ. (Taiwan)

Published in SPIE Proceedings Vol. 7762:
Optical Trapping and Optical Micromanipulation VII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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