Destructive fat tissue engineering using photodynamic and photothermal action is investigated in vitro. Samples of fat tissue were processed by a photosensitizer indocyanine green (ICG) and irradiated by a diode laser (805 nm). Computational methods of photo analysis are proposed for the quantitative estimation of the efficiency of actions on the cellular level. Techniques based on the fractal dimension, a segmentation method and statistical data analysis of fat cell structure during therapy are considered. The conducted experiments confirm the feasibility of the proposed methods. Results allow one to conclude that the proposed method of image processing of digital photos is a promising tool in estimation of structural changes of biological objects.

Date Published: 11 February 2010
PDF: 8 pages
Proc. SPIE 7547, Saratov Fall Meeting 2009: International School for Junior Scientists and Students on Optics, Laser Physics, and Biophotonics, 754708 (11 February 2010); doi: 10.1117/12.855988

Published in SPIE Proceedings Vol. 7547:
Saratov Fall Meeting 2009: International School for Junior Scientists and Students on Optics, Laser Physics, and Biophotonics
Valery V. Tuchin; Elina A. Genina, Editor(s)