
Proceedings Paper
A microfluidic system for semicontinuous bead-based biosensing platformsFormat | Member Price | Non-Member Price |
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Paper Abstract
Conventional bead-based micro immunoassays do not guarantee high reliability and repeatability due to the trapping
mechanisms of the systems which cause inconsistency in trapping of cytometric beads. This paper presents a new beadbased
micro immunoassay system for semicontinuous assays with high reliability and accuracy in cytometric bead
trapping by using the hole-patterned microstructures. Trapping test showed the assembly of single layer of bead array in
ordered fashion. In addition that, the μ-immunoassay exhibits that the fluorescence intensity linearly increases in
proportion to Biotin-4-Fluorescin (B4F) concentration and a 10 pg/mL appears to be the lowest detectable concentration
for B4F after incubation time of 5 minutes.
Paper Details
Date Published: 25 November 2009
PDF: 6 pages
Proc. SPIE 7634, Optical Sensors and Biophotonics, 76340I (25 November 2009); doi: 10.1117/12.853098
Published in SPIE Proceedings Vol. 7634:
Optical Sensors and Biophotonics
Xingde Li; Qingming Luo; Vasilis Ntziachristos; Yoshiaki Yasuno, Editor(s)
PDF: 6 pages
Proc. SPIE 7634, Optical Sensors and Biophotonics, 76340I (25 November 2009); doi: 10.1117/12.853098
Show Author Affiliations
Hye Won Kim, Gwangju Institute of Science and Technology (Korea, Republic of)
Young Man Kim, Gwangju Institute of Science and Technology (Korea, Republic of)
Sung Keun Yoo, Gwangju Institute of Science and Technology (Korea, Republic of)
Young Man Kim, Gwangju Institute of Science and Technology (Korea, Republic of)
Sung Keun Yoo, Gwangju Institute of Science and Technology (Korea, Republic of)
Sang Youl Yoon, Gwangju Institute of Science and Technology (Korea, Republic of)
Jong Hyun Lee, Gwangju Institute of Science and Technology (Korea, Republic of)
Sung Yang, Gwangju Institute of Science and Technology (Korea, Republic of)
Jong Hyun Lee, Gwangju Institute of Science and Technology (Korea, Republic of)
Sung Yang, Gwangju Institute of Science and Technology (Korea, Republic of)
Published in SPIE Proceedings Vol. 7634:
Optical Sensors and Biophotonics
Xingde Li; Qingming Luo; Vasilis Ntziachristos; Yoshiaki Yasuno, Editor(s)
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