The identification of pesticide and 6-benzylaminopurine (6-BAP) plant growth regulator was carried out using a label-free opto-fluidic ring resonator (OFRR) biosensor. The OFRR sensing platform is a recent advancement in opto-fluidic technology that integrates photonic sensing technology with microfluidics. It features quick detection time, small sample volume, accurate quantitative and kinetic results. The most predominant advantage of the OFRR integrated with microfluidics is that we can potentially realize the multi-channel and portable biosensor that detects numerous analytes simultaneously. Antisera for immunoassay were raised in rabbits against the 6-BAP-BSA conjugate. Using the immunization protocol and unknown cytokinin reacting with same antibody, comparable sensitivity and specificity were obtained. 6-BAP antibody was routinely used for cytokinin analysis. A sensitive and simple OFRR method with a good linear relationship was developed for the determination of 6-BAP. The detection limit was also examined. The biosensor demonstrated excellent reproducibility when periodically exposed to 6-BAP.

Date Published: 20 April 2010
PDF: 8 pages
Proc. SPIE 7676, Sensing for Agriculture and Food Quality and Safety II, 76760L (20 April 2010); doi: 10.1117/12.852096

Published in SPIE Proceedings Vol. 7676:
Sensing for Agriculture and Food Quality and Safety II
Moon S. Kim; Shu-I Tu; Kaunglin Chao, Editor(s)