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Proceedings Paper

In vivo measurement of Indocyanine green biodistribution in mammalian organs using fiber based system
Author(s): Qixiao Chen; Shuo Mao; Jing Bai
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Paper Abstract

Indocyanine green (ICG) is a fluorescent probe widely used in recent years, and it is also the fluorescent dye that can be clinical used, in both imaging and treatment. So it is important to study its biodistribution and metabolism in mammalian organs, but the accuracy and sampling speed is limited by the traditional in-vitro methods. Now we present a design of an in-vivo multi-channel fluorescence intensity measurement system and an algorism of data processing, to achieve the accurate measurement of fluorescence intensity, continuous sampling, real time monitoring and curve fitting. This system design is based on customized fiber bundles and the principle of reflective fluorescence microscopy. We also present a mouse experiment using this system to study the Indocyanine green (ICG) biodistribution in small mammalian liver, in order to demonstrate the potential applications of this system and also present a new experiment method in the study of dye biodistribution and metabolism.

Paper Details

Date Published: 25 November 2009
PDF: 6 pages
Proc. SPIE 7634, Optical Sensors and Biophotonics, 76340C (25 November 2009); doi: 10.1117/12.851851
Show Author Affiliations
Qixiao Chen, Tsinghua Univ. (China)
Shuo Mao, Tsinghua Univ. (China)
Jing Bai, Tsinghua Univ. (China)

Published in SPIE Proceedings Vol. 7634:
Optical Sensors and Biophotonics
Xingde Li; Qingming Luo; Vasilis Ntziachristos; Yoshiaki Yasuno, Editor(s)

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