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Proceedings Paper

Fabrication and characterization of mono-layered polystyrene beads using nanosphere lithography (NSL) for metal-enhanced fluorescence (MEF)
Author(s): Ravi Kumar Kannadorai; U. S Dinish; Chit Yaw Fu; Malini Olivo; Anand Asundi
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Paper Abstract

Fluorescence spectroscopy is a powerful and widely used technique, which finds extensive applications in biochemistry and molecular biology. In many cases, detection of fluorescence from biological samples at low concentration demands for a sensitive technique that can enhance the weak fluorescence signal. In order to realize this, metal enhanced fluorescence technique is employed where an increase in fluorescence is observed when the fluorophore is in close proximity to a metallic surface. In order to achieve this, nano-roughened silver surfaces such as fractal structures or silver colloid-coated surfaces can be employed. However, preparation of such surfaces not only involves lengthy chemical procedures but also result in poor reproducibility. To overcome these limitations, nanosphere lithography (NSL) is proposed, which is an inexpensive, simple to implement and high throughput nanofabrication technique. In this technique, polystyrene (PS) nano-beads are used to form a 2D monolayer of nanoparticle array followed by deposition of silver to form a roughened metallic surface. The surface roughness of the silver coating is determined by the close packing and arrangement of the nanobeads. In this work, substrates are fabricated using different sizes of PS beads to result in different nanometric surface roughening for silver layer and its optimization to achieve better fluorescence enhancement is carried out.

Paper Details

Date Published: 14 April 2010
PDF: 6 pages
Proc. SPIE 7522, Fourth International Conference on Experimental Mechanics, 752269 (14 April 2010); doi: 10.1117/12.851636
Show Author Affiliations
Ravi Kumar Kannadorai, Nanyang Technological Univ. (Singapore)
U. S Dinish, Singapore Bioimaging Consortium, A*STAR Agency for Science and Technology (Singapore)
Chit Yaw Fu, Singapore Bioimaging Consortium, A*STAR Agency for Science and Technology (Singapore)
Malini Olivo, Singapore Bioimaging Consortium, A*STAR Agency for Science and Technology (Singapore)
Anand Asundi, Nanyang Technological Univ. (Singapore)

Published in SPIE Proceedings Vol. 7522:
Fourth International Conference on Experimental Mechanics
Chenggen Quan; Kemao Qian; Anand Krishna Asundi; Fook Siong Chau, Editor(s)

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