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Proceedings Paper

New strategies to measure intracellular sodium concentrations
Author(s): Sascha Dietrich; Sarmiza E. Stanca; Charles G. Cranfield; Birgit Hoffmann; Klaus Benndorf; Christoph Biskup
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Paper Abstract

Fluorescent ion indicators are widely used to measure ion concentrations in living cells. However, despite considerable efforts in synthesizing new compounds, no ratiometric sodium indicator is available that can be excited at visible wavelengths. Ratiometric indicators have an advantage in that measured fluorescence intensities can be corrected for fluctuations of the indicator concentration and the illumination intensity, which is not possible when non-ratiometric indicators are used. One way to circumvent this problem is to measure fluorescence lifetimes, which are independent of these factors. Another way to overcome the disadvantages of a non-ratiometric indicator dye is to embed it, together with a reference dye, into nanoparticles. By relating the indicator fluorescence to the fluorescence of the reference dye, inhomogeneities in the nanosensor concentration or the illumination intensity can be cancelled out reliably. In this study we compare the benefits and drawbacks of these approaches.

Paper Details

Date Published: 2 March 2010
PDF: 9 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756914 (2 March 2010); doi: 10.1117/12.849742
Show Author Affiliations
Sascha Dietrich, Univ. Hospital Jena (Germany)
Sarmiza E. Stanca, Univ. Hospital Jena (Germany)
Charles G. Cranfield, Univ. Hospital Jena (Germany)
Birgit Hoffmann, Univ. Hospital Jena (Germany)
Klaus Benndorf, Univ. Hospital Jena (Germany)
Christoph Biskup, Univ. Hospital Jena (Germany)

Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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