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Proceedings Paper

Portable, battery-operated, fluorescence field microscope for the developing world
Author(s): Andrew R. Miller; Gregory Davis; Mark Pierce; Z. Maria Oden; Rebecca Richards-Kortum
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Paper Abstract

In many areas of the world, current methods for diagnosis of infectious diseases such as malaria and tuberculosis involve microscopic evaluation of a patient specimen. Advances in fluorescence microscopy can improve diagnostic sensitivity and reduce time and expertise necessary to interpret diagnostic results. However, modern research-grade microscopes are neither available nor appropriate for use in many settings in the developing world. To address this need, we designed, fabricated, and tested a portable, battery-powered, bright field and fluorescence inverted field microscope, optimized for infrastructural constraints of the developing world. We characterized an initial prototype constructed with rapidprototyping techniques, which utilized low-cost, over-the-counter components such as a battery-powered LED flashlight as the light source. The microscope exhibited suitable spatial resolution (0.8 μm) in fluorescence mode to resolve M. tuberculosis bacilli. In bright field mode, malaria parasites were resolvable at 1000x magnification. The initial prototype cost 480 USD and we estimate that the microscope can be manufactured for 230 USD. While future studies are planned to evaluate ease-of-use and reliability, our current system serves as a proof of concept that combined fluorescence and bright field microscopy is possible in a low-cost and portable system.

Paper Details

Date Published: 23 February 2010
PDF: 7 pages
Proc. SPIE 7556, Design and Quality for Biomedical Technologies III, 755608 (23 February 2010); doi: 10.1117/12.848605
Show Author Affiliations
Andrew R. Miller, Rice Univ. (United States)
Gregory Davis, Rice Univ. (United States)
Mark Pierce, Rice Univ. (United States)
Z. Maria Oden, Rice Univ. (United States)
Rebecca Richards-Kortum, Rice Univ. (United States)

Published in SPIE Proceedings Vol. 7556:
Design and Quality for Biomedical Technologies III
Ramesh Raghavachari; Rongguang Liang, Editor(s)

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