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Proceedings Paper

Nonequilibrium fluctuations in metaphase spindles: polarized light microscopy, image registration, and correlation functions
Author(s): Jan Brugués; Daniel J. Needleman
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Paper Abstract

Metaphase spindles are highly dynamic, nonequilibrium, steady-state structures. We study the internal fluctuations of spindles by computing spatio-temporal correlation functions of movies obtained from quantitative polarized light microscopy. These correlation functions are only physically meaningful if corrections are made for the net motion of the spindle. We describe our image registration algorithm in detail and we explore its robustness. Finally, we discuss the expression used for the estimation of the correlation function in terms of the nematic order of the microtubules which make up the spindle. Ultimately, studying the form of these correlation functions will provide a quantitative test of the validity of coarse-grained models of spindle structure inspired from liquid crystal physics.

Paper Details

Date Published: 12 February 2010
PDF: 10 pages
Proc. SPIE 7618, Emerging Liquid Crystal Technologies V, 76180L (12 February 2010); doi: 10.1117/12.848223
Show Author Affiliations
Jan Brugués, Harvard Univ. (United States)
Daniel J. Needleman, Harvard Univ. (United States)


Published in SPIE Proceedings Vol. 7618:
Emerging Liquid Crystal Technologies V
Liang-Chy Chien, Editor(s)

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