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Proceedings Paper

Assessment of microcirculation frequency components through functional diffuse reflectance spectroscopy: a promising tool for noninvasive skin diagnosis
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Paper Abstract

Measurement of cutaneous microcirculation is of great importance for clinical evaluations as many biological processes (i.e. inflammation) activate superficial vessels. Diffuse Reflectance Spectroscopy (DRS) is a widely used method to assess cutaneous microcirculation since it is based on the spectral characteristics of skin chromophores, particularly the strong absorption bands of blood in the visible spectral range. However, the DRS system only provides a snapshot of the blood content in tissue from the reflectance detected at a single exposure time, and it fails to demonstrate dynamic changes of blood flow inside the tissues. In the present study, a prototype system for functional DRS (fDRS) has been developed for the sequential acquisition of multiple reflectance spectra in the visible range at sub-second intervals. Twelve healthy subjects with skin phototype I-III were recruited for a UV skin phototest where the subject's back was exposed to an increasing dose of solar-simulated radiation at a maximum of 3 minimum erythema dose (MED). Evaluations included conventional DRS, functional DRS, and a laser Doppler flowmeter/imaging system. In a frequency analysis results, there were two distinctive frequency components. A low-frequency component was found near 0.03-0.1 Hz, and another high-frequency component near 0.9-1.2 Hz which is synchronous with heart pulsations. The magnitude of the high-frequency and the steady-state components of the fDRS signal increased with increase in exposure dose. These results demonstrate the potential of this technique for noninvasive assessment of cutaneous microcirculation.

Paper Details

Date Published: 2 March 2010
PDF: 5 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75480O (2 March 2010); doi: 10.1117/12.848217
Show Author Affiliations
Paulo R. Bargo, Johnson & Johnson CPPW (United States)
InSeok Seo, Johnson & Johnson CPPW (United States)
Nikiforos Kollias, Johnson & Johnson CPPW (United States)

Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong M.D.; Nikiforos Kollias; Henry Hirschberg M.D.; Kenton W. Gregory M.D.; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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