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Proceedings Paper

Design and fabrication of a PDMS microchip based immunoassay
Author(s): Guocheng Shao; Wanjun Wang; Jun Wang; Yuehe Lin
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Paper Abstract

In this paper, we describe the design and fabrication of a polydimethylsiloxane (PDMS) microchip for on-chip multiplex immunoassay applications. The microchip consists of a PDMS microfluidic channel layer and a micro pneumatic valve control layer. By selectively pressurizing the pneumatic microvalves, immuno reagents were controlled to flow and react in certain fluidic channel sites. Cross contamination was prevented by tightly closed valves. Our design was proposed to utilize PDMS micro channel surface as the solid phase immunoassay substrate and simultaneously detect four targets antigens on chip. Experimental results show that 20psi valve pressure is sufficient to tightly close a 200μm wide micro channel with flow rate up to 20μl/min.

Paper Details

Date Published: 17 February 2010
PDF: 6 pages
Proc. SPIE 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII, 75930Q (17 February 2010); doi: 10.1117/12.847138
Show Author Affiliations
Guocheng Shao, Louisiana State Univ. (United States)
Wanjun Wang, Louisiana State Univ. (United States)
Jun Wang, Pacific Northwest National Lab. (United States)
Yuehe Lin, Pacific Northwest National Lab. (United States)

Published in SPIE Proceedings Vol. 7593:
Microfluidics, BioMEMS, and Medical Microsystems VIII
Holger Becker; Wanjun Wang, Editor(s)

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