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Proceedings Paper

Near infrared Raman spectroscopic study of reactive gliosis and the glial scar in injured rat spinal cords
Author(s): Tarun Saxena; Bin Deng; Eric Lewis-Clark; Kyle Hoellger; Dennis Stelzner; Julie Hasenwinkel; Joseph Chaiken
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Paper Abstract

Comparative Raman spectra of ex vivo, saline-perfused, injured and healthy rat spinal cord as well as experiments using enzymatic digestion suggest that proteoglycan over expression may be observable in injured tissue. Comparison with authentic materials in vitro suggest the occurrence of side reactions between products of cord digestion with chondroitinase (cABC) that produce lactones and similar species with distinct Raman features that are often not overlapped with Raman features from other chemical species. Since the glial scar is thought to be a biochemical and physical barrier to nerve regeneration, this observation suggests the possibility of using near infrared Raman spectroscopy to study disease progression and explore potential treatments ex vivo and if potential treatments can be designed, perhaps to monitor potential remedial treatments within the spinal cord in vivo.

Paper Details

Date Published: 25 February 2010
PDF: 12 pages
Proc. SPIE 7560, Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry, 75600I (25 February 2010); doi: 10.1117/12.846897
Show Author Affiliations
Tarun Saxena, Syracuse Univ. (United States)
Bin Deng, Syracuse Univ. (United States)
Eric Lewis-Clark, SUNY Binghamton (United States)
Kyle Hoellger, SUNY Binghamton (United States)
Dennis Stelzner, SUNY Upstate Medical Univ. (United States)
Julie Hasenwinkel, Syracuse Univ. (United States)
Joseph Chaiken, SUNY Upstate Medical Univ. (United States)

Published in SPIE Proceedings Vol. 7560:
Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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