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Proceedings Paper

A PDMS sample pretreatment microdevice to enable downstream electrokinetic manipulations in bovine serum
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Paper Abstract

The notion of sample preconditioning, or pretreatment, as a micro-unit operation in a Lab on a Chip (LOC) system has yet to be realized in commercial practice. As is well known, Biomarker detection in complex, biological samples, such as blood, requires a series of pretreatment steps to enable detection of specific markers. On chip, such a process usually relies on "off-chip" sample pretreatment prior to "on-chip" analyte manipulations and detection. Presented in this paper is a PDMS, pretreatment chip based on the design of Oddy et al.1 with a view to enable a self-contained LOC platform. The chip was designed to directly manipulate the suspended species while adjusting fluid properties using buffer volumes less than 1 ml. Using previous literature related to capillary electrophoresis, a bench-scale pretreatment protocol was developed to tune specific fluidic parameters to an optimal range, namely pH, conductivity, and viscosity. A PDMS device was fabricated and used to combine a raw, bovine serum sample with specific buffer solutions. Off-chip electrodes were used to induce DC-electrokinetic micro-mixing of the target analyte in the mixing chamber, where a homogeneous analyte distribution was achieved in less than one second using an 800V DC pulse wave. Additionally, the desired solution viscosity and pH were achieved using less than 1 ml of buffer solution. Adjustment of sample conductivity, which is driven by sample fluid volume, remains an open area of research.

Paper Details

Date Published: 17 February 2010
PDF: 12 pages
Proc. SPIE 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII, 759318 (17 February 2010); doi: 10.1117/12.846448
Show Author Affiliations
Timothy J. Abram, California Polytechnic State Univ., San Luis Obispo (United States)
David S. Clague, California Polytechnic State Univ., San Luis Obispo (United States)

Published in SPIE Proceedings Vol. 7593:
Microfluidics, BioMEMS, and Medical Microsystems VIII
Holger Becker; Wanjun Wang, Editor(s)

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