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Proceedings Paper

Implementation of adaptive optics in fluorescent microscopy using wavefront sensing and correction
Author(s): Oscar Azucena; Justin Crest; Jian Cao; William Sullivan; Peter Kner; Donald Gavel; Daren Dillon; Scot Olivier; Joel Kubby
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Paper Abstract

Adaptive optics (AO) improves the quality of astronomical imaging systems by using real time measurement of the turbulent medium in the optical path using a guide star (natural or artificial) as a point source reference beacon [1]. AO has also been applied to vision science to improve the view of the human eye. This paper will address our current research focused on the improvement of fluorescent microscopy for biological imaging utilizing current AO technology. A Shack-Hartmann wavefront sensor (SHWS) is used to measure the aberration introduced by a Drosophila Melanogaster embryo with an implanted 1 micron fluorescent bead that serves as a point source reference beacon. Previous measurements of the wavefront aberrations have found an average peak-to-valley and root-mean-square (RMS) wavefront error of 0.77 micrometers and 0.15 micrometers, respectively. Measurements of the Zernike coefficients indicated that the correction of the first 14 Zernike coefficients is sufficient to correct the aberrations we measured. Here we show that a MEMS deformable mirror with 3.5 microns of stroke and 140 actuators is sufficient to correct these aberrations. The design, assembly and initial results for the use of a MEMS deformable mirror, SHWS and implanted fluorescent reference beacon for wavefront correction are discussed.

Paper Details

Date Published: 18 February 2010
PDF: 9 pages
Proc. SPIE 7595, MEMS Adaptive Optics IV, 75950I (18 February 2010); doi: 10.1117/12.846380
Show Author Affiliations
Oscar Azucena, Univ. of California, Santa Cruz (United States)
Justin Crest, Univ. of California, Santa Cruz (United States)
Jian Cao, Univ. of California, Santa Cruz (United States)
William Sullivan, Univ. of California, Santa Cruz (United States)
Peter Kner, Univ. of California, San Francisco (United States)
Donald Gavel, Univ. of California Observatories (United States)
Daren Dillon, Univ. of California Observatories (United States)
Scot Olivier, Lawrence Livermore National Lab. (United States)
Joel Kubby, Univ. of California, Santa Cruz (United States)

Published in SPIE Proceedings Vol. 7595:
MEMS Adaptive Optics IV
Scot S. Olivier; Thomas G. Bifano; Joel A. Kubby, Editor(s)

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