We have developed an adaptive optics multiphoton microscope. The multiphoton imaging system combines an ultrafast high-power laser, a scanning unit, a motorized Z-scan device and a photon-counting detector. The adaptive optics module is composed of a Hartmann-Shack wavefront sensor and a MEMS deformable mirror. The impact of compensating the aberrations of the laser beam is shown in a number of biological and non-biological samples. As examples, nonlinear fluorescence and second harmonic generation images of non-stained ex-vivo ocular tissues are compared with and without adaptive optics. The correction of the beam's aberrations increases both contrast and resolution in the non-linear microscope images.

Date Published: 18 February 2010
PDF: 6 pages
Proc. SPIE 7595, MEMS Adaptive Optics IV, 75950G (18 February 2010); doi: 10.1117/12.845981

Published in SPIE Proceedings Vol. 7595:
MEMS Adaptive Optics IV
Scot S. Olivier; Thomas G. Bifano; Joel A. Kubby, Editor(s)