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Proceedings Paper

A multimodal multiphoton microscope for biological imaging
Author(s): Rabah Mouras; Andrew Downes; Grigore Rischitor; Meropi Mari; Alistair Elfick
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Paper Abstract

We report on the construction of a highly flexible system for advanced biological imaging, where all the following imaging techniques are integrated into the same microscope: Coherent anti-Stokes Raman scattering (CARS), two photon excitation fluorescence (TPEF), second harmonic generation (SGH), sum frequency generation (SFG), fluorescence lifetime imaging (FLIM) and differential interference contrast (DIC). The system employs a Nd:YVO4 laser as pump (7 ps, 1064 nm), and two tunable OPOs (6 ps, 700 - 1000 nm). Our microscope comprises a heater stage and perfusion cell for imaging of live cells, and features an atomic force microscope (AFM) which enables optical imaging at 10 nm resolution. Multimodal imaging of breast cancer cells and tissue will be demonstrated as well as imaging of anticancer drugs in living cells.

Paper Details

Date Published: 26 February 2010
PDF: 6 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756933 (26 February 2010); doi: 10.1117/12.844236
Show Author Affiliations
Rabah Mouras, Univ. of Edinburgh (United Kingdom)
Andrew Downes, Univ. of Edinburgh (United Kingdom)
Grigore Rischitor, Univ. of Edinburgh (United Kingdom)
Meropi Mari, Univ. of Edinburgh (United Kingdom)
Alistair Elfick, Univ. of Edinburgh (United Kingdom)

Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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