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Proceedings Paper

Raman spectroscopic analysis of cytotoxic effect of cisplatin-treated leukemic cells
Author(s): Juqiang Lin; Yongzeng Li; Shangyuan Feng; Rong Chen; Guannan Chen; Qisong Chen; Jianji Pan; Shaojun Lin; Yun Yu
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Paper Abstract

An antitumor drug cisplatin was employed to treat the leukemic cells and induce apoptosis of the cancer cells. Confocal Raman micro-spectroscopy has been applied to investigate the effectiveness of the treatment using near-infrared laser (785nm) excitation, scanning range from 500 to 2000 cm-1. The Raman spectra of leukemic cell treated with cisplatin for 4, 6, 8, 12 and 14 h were measured separately. The major difference of the apoptotic cells from the cancer cells are the reduction in intensities of vibration bands generated by cellular lipids, proteins and nucleic acids. In particular, large intensity reduction in nucleic vibrations at 782, 1092, 1320, 1340, and 1578 cm-1 was observed upon apoptosis of the leukemic cells. Up to 45% reduction in the magnitude of the 782 cm-1 peak in Raman spectra of the apoptotic cells was observed, which suggests the breakdown of phosphodiester bonds and DNA bases. We showed that the principal components analysis (PCA), a multivariate statistical tool, can be used to distinguish single apoptotic cells and leukemic cells based on their Raman spectra. Our results indicate that the Raman spectroscopy with PCA is a novel, nondestructive mean for studying the cisplatin -treated leukemic cells, which could also provide useful data for clinical dosage optimization for cisplatin.

Paper Details

Date Published: 28 October 2009
PDF: 5 pages
Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75191G (28 October 2009); doi: 10.1117/12.844042
Show Author Affiliations
Juqiang Lin, Fujian Normal Univ. (China)
Yongzeng Li, Fujian Normal Univ. (China)
Shangyuan Feng, Fujian Normal Univ. (China)
Rong Chen, Fujian Normal Univ. (China)
Xiamen Univ. (China)
Guannan Chen, Fujian Normal Univ. (China)
Qisong Chen, Fujian Provincial Tumor Hospital (China)
Jianji Pan, Fujian Provincial Tumor Hospital (China)
Shaojun Lin, Fujian Provincial Tumor Hospital (China)
Yun Yu, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 7519:
Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Pengcheng Li; Ling Fu, Editor(s)

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