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Proceedings Paper

In vivo multi-modality photoacoustic and pulse echo tracking of prostate tumor growth using a window chamber
Author(s): Daniel R. Bauer; Ragnar Olafsson; Leonardo G. Montilla; Russell S. Witte
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Paper Abstract

Understanding the tumor microenvironment is critical to characterizing how cancers operate and predicting how they will eventually respond to treatment. The mouse window chamber model is an excellent tool for cancer research, because it enables high resolution tumor imaging and cross-validation using multiple modalities. We describe a novel multimodality imaging system that incorporates three dimensional (3D) photoacoustics with pulse echo ultrasound for imaging the tumor microenvironment and tracking tissue growth in mice. Three mice were implanted with a dorsal skin flap window chamber. PC-3 prostate tumor cells, expressing green fluorescent protein (GFP), were injected into the skin. The ensuing tumor invasion was mapped using photoacoustic and pulse echo imaging, as well as optical and fluorescent imaging for comparison and cross validation. The photoacoustic imaging and spectroscopy system, consisting of a tunable (680-1000nm) pulsed laser and 25 MHz ultrasound transducer, revealed near infrared absorbing regions, primarily blood vessels. Pulse echo images, obtained simultaneously, provided details of the tumor microstructure and growth with 100-μm3 resolution. The tumor size in all three mice increased between three and five fold during 3+ weeks of imaging. Results were consistent with the optical and fluorescent images. Photoacoustic imaging revealed detailed maps of the tumor vasculature, whereas photoacoustic spectroscopy identified regions of oxygenated and deoxygenated blood vessels. The 3D photoacoustic and pulse echo imaging system provided complementary information to track the tumor microenvironment, evaluate new cancer therapies, and develop molecular imaging agents in vivo. Finally, these safe and noninvasive techniques are potentially applicable for human cancer imaging.

Paper Details

Date Published: 23 February 2010
PDF: 10 pages
Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75643B (23 February 2010); doi: 10.1117/12.843875
Show Author Affiliations
Daniel R. Bauer, The Univ. of Arizona (United States)
Ragnar Olafsson, The Univ. of Arizona (United States)
Leonardo G. Montilla, The Univ. of Arizona (United States)
Russell S. Witte, The Univ. of Arizona (United States)

Published in SPIE Proceedings Vol. 7564:
Photons Plus Ultrasound: Imaging and Sensing 2010
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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