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Proceedings Paper

Adaptive multiphoton and harmonic generation microscopy for developmental biology
Author(s): Anisha Thayil; Alexander Jesacher; Tomoko Watanabe; Tony Wilson; Shankar Srinivas; Martin J. Booth
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Paper Abstract

Specimen-induced aberrations often affect microscopes, particularly when high numerical aperture lenses are used to image deep into biological specimens. These aberrations cause a reduction in resolution and focal intensity. This is particularly problematic in multiphoton microscopy, where the non-linear nature of the signal generation process means that the signal level is strongly affected by changes in the focal spot intensity. We have applied the techniques of adaptive optics to correct aberrations in two-photon fluorescence and harmonic generation microscopes, restoring image quality. In particular we have used these microscopes for studies in developmental biology and for the imaging of mammalian embryos.

Paper Details

Date Published: 26 February 2010
PDF: 6 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75691I (26 February 2010); doi: 10.1117/12.843312
Show Author Affiliations
Anisha Thayil, Univ. of Oxford (United Kingdom)
Alexander Jesacher, Univ. of Oxford (United Kingdom)
Tomoko Watanabe, Univ. of Oxford (United Kingdom)
Tony Wilson, Univ. of Oxford (United Kingdom)
Shankar Srinivas, Univ. of Oxford (United Kingdom)
Martin J. Booth, Univ. of Oxford (United Kingdom)

Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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