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Proceedings Paper

Human hepatocellular carcinoma diagnosis by multiphoton autofluorescence microscopy
Author(s): Tzu-Lin Sun; Yuan Liu; Ming-Chin Sung; Hsiao-Ching Chen; Chuen-Huei Yang; Vladimir Hovhannisyan; Ling-Ling Chiou; Wei-Chou Lin; Guan-Tarn Huang; Ki-Hean Kim; Peter T. C. So; Chih-Ju Lin; Hsuan-Shu Lee; Chen-Yuan Dong
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Paper Abstract

Conventionally, the diagnosis of hepatocellular carcinoma (HCC) is performed by qualitative examination of histopathological specimens, which takes times for sample preparation in fixation, section and stain. Our objective is to demonstrate an effective and efficient approach to apply multiphoton microscopy imaging the HCC specimens, with the advantages of being optical section, label-free, subcellular resolution, minimal invasiveness, and the acquisition of quantitative information at the same time. The imaging modality of multiphoton autofluorescence (MAF) was used for the qualitative imaging and quantitative analysis of HCC of different grades under ex-vivo, label-free conditions. We found that while MAF is effective in identifying cellular architecture in the liver specimens, and obtained quantitative parameters in characterizing the disease. Our results demonstrates the capability of using tissue quantitative parameters of multiphoton autofluorescence (MAF), the nuclear number density (NND), and nuclear-cytoplasmic ratio (NCR) for tumor discrimination and that this technology has the potential in clinical diagnosis of HCC and the in-vivo investigation of liver tumor development in animal models.

Paper Details

Date Published: 23 February 2010
PDF: 9 pages
Proc. SPIE 7555, Advanced Biomedical and Clinical Diagnostic Systems VIII, 75551L (23 February 2010); doi: 10.1117/12.843157
Show Author Affiliations
Tzu-Lin Sun, National Taiwan Univ. (Taiwan)
Yuan Liu, National Taiwan Univ. (Taiwan)
Ming-Chin Sung, National Taiwan Univ. (Taiwan)
Hsiao-Ching Chen, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Chuen-Huei Yang, National Taiwan Univ. (Taiwan)
Vladimir Hovhannisyan, National Taiwan Univ. (Taiwan)
Ling-Ling Chiou, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Wei-Chou Lin, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Guan-Tarn Huang, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Ki-Hean Kim, Massachusetts Institute of Technology (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)
Chih-Ju Lin, National Taiwan Univ. (Taiwan)
Hsuan-Shu Lee, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 7555:
Advanced Biomedical and Clinical Diagnostic Systems VIII
Tuan Vo-Dinh; Warren S. Grundfest M.D.; Anita Mahadevan-Jansen, Editor(s)

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