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Proceedings Paper

Structural and biochemical characterization of the rat retina with combined Raman spectroscopy-spectral domain optical coherence tomography (RS-SDOCT)
Author(s): Chetan A. Patil; Jeroen Kalkman; Dirk Faber; John S. Penn; Ton G. van Leeuwen; Anita Mahadevan-Jansen
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Paper Abstract

Raman spectroscopy (RS) and Optical Coherence Tomography (OCT) are powerful tools for optical analysis of tissues with complimentary strengths. OCT excels and visualizing tissue microstructure while RS can relay tissue biochemical composition. Both instruments have significant potential to serve as valuable tools in non-invasive characterization of the rodent models of retinal disease. In this abstract we present the design and application of a common detector combined RS-OCT instrument for evaluating the morphological and biochemical differences in a rat model for oxygen induced retinopathy. Rat pups that have undergone a variable oxygen treatment are compared to rats raised in room air. Images and spectra collected at an age of 26 days postnatal demonstrate differences in both the thickness of the inner and outer nuclear layers, but also in the biochemical composition.

Paper Details

Date Published: 2 March 2010
PDF: 6 pages
Proc. SPIE 7550, Ophthalmic Technologies XX, 75501B (2 March 2010); doi: 10.1117/12.843106
Show Author Affiliations
Chetan A. Patil, Vanderbilt Univ. (United States)
Jeroen Kalkman, Amsterdam Medical Ctr. (Netherlands)
Dirk Faber, Amsterdam Medical Ctr. (Netherlands)
John S. Penn, Vanderbilt Univ. Medical Ctr. (United States)
Ton G. van Leeuwen, Amsterdam Medical Ctr. (Netherlands)
Anita Mahadevan-Jansen, Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 7550:
Ophthalmic Technologies XX
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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