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Proceedings Paper

Second-order susceptibility imaging with polarization-resolved second harmonic generation microscopy
Author(s): Wei-Liang Chen; Tsung-Hsian Li; Ping-Jung Su; Chen-Kuan Chou; Peter Tramyeon Fwu; Sung-Jan Lin; Daekeun Kim; Peter T. C. So; Chen-Yuan Dong
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Paper Abstract

Second harmonic generation (SHG) microscopy has become an important tool for minimally invasive biomedical imaging. However, differentiation of different second harmonic generating species is mainly provided by morphological features. Using excitation polarization-resolved SHG microscopy we determined the ratios of the second-order susceptibility tensor elements at single pixel resolution. Mapping the resultant ratios for each pixel onto an image provides additional contrast for the differentiation of different sources of SHG. We demonstrate this technique by imaging collagen-muscle junction of chicken wing.

Paper Details

Date Published: 26 February 2010
PDF: 7 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75691P (26 February 2010); doi: 10.1117/12.843015
Show Author Affiliations
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Tsung-Hsian Li, National Taiwan Univ. (Taiwan)
Ping-Jung Su, National Taiwan Univ. (Taiwan)
Chen-Kuan Chou, National Taiwan Univ. (Taiwan)
Peter Tramyeon Fwu, National Taiwan Univ. (Taiwan)
Sung-Jan Lin, National Taiwan Univ. Hospital (Taiwan)
Daekeun Kim, Massachusetts Institute of Technology (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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