
Proceedings Paper
Multiplex coherent anti-Stokes Raman scattering flow cytometry for real-time classification of particles in a microfluidic channelFormat | Member Price | Non-Member Price |
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Paper Abstract
Flow cytometry is an ever-advancing, multivariate analysis tool obtaining morphological and phenotype information
from sample populations. In traditional flow cytometers, obtaining molecular information requires the
use of endogenous fluorophores, which are limited by spectral overlap, nonspecific binding, available conjugation
chemistries, and cellular toxicity. In this work, however, we apply multiplex coherent anti-Stokes Raman scattering
(MCARS), a nonlinear optical method that probes the Raman energies within a molecule, to flow cytometry
in order to demonstrate label-free, molecularly sensitive particle differentiation. To demonstrate this, we correctly
differentiate 5 μm polystyrene (PS) and poly(methyl methacrylate) (PMMA), which (linearly) optically
appear similar, but vary significantly in their MCARS spectrum.
Paper Details
Date Published: 26 February 2010
PDF: 7 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692F (26 February 2010); doi: 10.1117/12.843011
Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)
PDF: 7 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692F (26 February 2010); doi: 10.1117/12.843011
Show Author Affiliations
Charles H. Camp Jr., Georgia Institute of Technology (United States)
Siva Yegnanarayanan, Georgia Institute of Technology (United States)
Ali A. Eftekhar, Georgia Institute of Technology (United States)
Siva Yegnanarayanan, Georgia Institute of Technology (United States)
Ali A. Eftekhar, Georgia Institute of Technology (United States)
Hamsa Sridhar, Harvard Univ. (United States)
Ali Adibi, Georgia Institute of Technology (United States)
Ali Adibi, Georgia Institute of Technology (United States)
Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)
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