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Proceedings Paper

Availability of fluorescence spectroscopic in the accompaniment of formation of corneal cross-linking
Author(s): M. M. Costa; C. Kurachi; V. S. Bagnato; S. J. Faria e Sousa; L. Ventura
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Paper Abstract

The corneal cross-linking is a method that associates riboflavin and ultraviolet light to induce a larger mechanical resistance at cornea. This method has been used for the treatment of Keratoconus. Since cross-linking is recent as treatment, there is a need to verify the effectiveness of the method. Therefore, the viability of the fluorescence spectroscopy technique to follow the cross-linking formation at cornea was studied. Corneas were divided in two measuring procedures: M1 (cornea + riboflavin), and M2 (cornea + riboflavina + light irradiation, 365nm). For fluorescence measurements, a spectrofluorimeter was used, where several wavelengths were selected (between 320nm and 370nm) for cornea excitation. Several fluorescence spectra were collected, at 10 min-interval, during 60 min. Spectra allowed one to observe two very well defined bands of fluorescence: the first one at 400nm (collagen), and the second one at 520nm (riboflavin). After spectra analyses, a decrease of collagen fluorescence was observed for both groups. For riboflavin, on the other hand, there was a fluorescence increase for M1, and a decrease for M2. Thus, it is possible to conclude that it this technique is sensitive for the detection of tissue structural changes during cross-linking treatment, encouraging subsequent studies on quantification of cross-linking promotion in tissue.

Paper Details

Date Published: 2 March 2010
PDF: 5 pages
Proc. SPIE 7550, Ophthalmic Technologies XX, 755023 (2 March 2010); doi: 10.1117/12.842940
Show Author Affiliations
M. M. Costa, Lab. de Instrumentação Oftálmica, EESC, Univ. de São Paulo (Brazil)
Lab. de Biofotônica, IFSC, Univ. de São Paulo (Brazil)
C. Kurachi, Lab. de Biofotônica, IFSC, Univ. de São Paulo (Brazil)
V. S. Bagnato, Lab. de Biofotônica, IFSC, Univ. de São Paulo (Brazil)
S. J. Faria e Sousa, FMRP, Univ. de São Paulo (Brazil)
L. Ventura, Lab. de Instrumentação Oftálmica, EESC, Univ. de São Paulo (Brazil)

Published in SPIE Proceedings Vol. 7550:
Ophthalmic Technologies XX
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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