
Proceedings Paper
Comparing coherent and spontaneous Raman scattering signals for biological imaging applicationsFormat | Member Price | Non-Member Price |
---|---|---|
$17.00 | $21.00 |
Paper Abstract
We present a systematic comparison between coherent and spontaneous Raman scattering under conditions
relevant for biological imaging. Using spectral domain imaging, we find that the signal levels for each method
are comparable at the low excitation power and low concentrations appropriate for biological samples. For
samples of polystyrene beads with a molecular concentration of 10 M, we determine the critical power at which
the two methods give equal signal levels to be ~1.3 mW. The advantages offered by coherent Raman methods
are mitigated by the low excitation power, low sample concentrations, and short interaction lengths involved
with biological imaging. We present calculations to support our measurements.
Paper Details
Date Published: 26 February 2010
PDF: 7 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75690O (26 February 2010); doi: 10.1117/12.842847
Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)
PDF: 7 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75690O (26 February 2010); doi: 10.1117/12.842847
Show Author Affiliations
Brandon R. Bachler, Univ. of Michigan (United States)
Meng Cui, Univ. of Michigan (United States)
Meng Cui, Univ. of Michigan (United States)
Sarah R. Nichols, Univ. of Michigan (United States)
Jennifer P. Ogilvie, Univ. of Michigan (United States)
Jennifer P. Ogilvie, Univ. of Michigan (United States)
Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)
© SPIE. Terms of Use
