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Proceedings Paper

Functional Doppler optical coherence tomography for cortical blood flow imaging
Author(s): Lingfeng Yu; Gangjun Liu; Elaine Nguyen; Bernard Choi; Zhongping Chen
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Paper Abstract

Optical methods have been widely used in basic neuroscience research to study the cerebral blood flow dynamics in order to overcome the low spatial resolution associated with magnetic resonance imaging and positron emission tomography. Although laser Doppler imaging and laser speckle imaging can map out en face cortical hemodynamics and columns, depth resolution is not available. Two-photon microscopy has been used for mapping cortical activity. However, flow measurement requires fluorescent dye injection, which can be problematic. The noninvasive and high resolution tomographic capabilities of optical coherence tomography make it a promising technique for mapping depth resolved cortical blood flow. Here, we present a functional Doppler optical coherence tomography (OCT) imaging modality for quantitative evaluation of cortical blood flow in a mouse model. Fast, repeated, Doppler OCT scans across a vessel of interest were performed to record flow dynamic information with a high temporal resolution of the cardiac cycles. Spectral Doppler analysis of continuous Doppler images demonstrates how the velocity components and longitudinally projected flow-volume-rate change over time, thereby providing complementary temporal flow information to the spatially distributed flow information of Doppler OCT. The proposed functional Doppler OCT imaging modality can be used to diagnose vessel stenosis/blockage or monitor blood flow changes due to pharmacological agents/neuronal activities. Non-invasive in-vivo mice experiments were performed to verify the capabilities of function Doppler OCT.

Paper Details

Date Published: 2 March 2010
PDF: 4 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75483Q (2 March 2010); doi: 10.1117/12.842820
Show Author Affiliations
Lingfeng Yu, Beckman Laser Institute and Medical Clinic, Univ. of California, Irvine (United States)
Gangjun Liu, Beckman Laser Institute and Medical Clinic, Univ. of California, Irvine (United States)
Elaine Nguyen, Beckman Laser Institute and Medical Clinic, Univ. of California, Irvine (United States)
Bernard Choi, Beckman Laser Institute and Medical Clinic, Univ. of California, Irvine (United States)
Zhongping Chen, Beckman Laser Institute and Medical Clinic, Univ. of California, Irvine (United States)


Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong M.D.; Nikiforos Kollias; Henry Hirschberg M.D.; Kenton W. Gregory M.D.; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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