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Proceedings Paper

Raman spectroscopic evidence of crystalline phosphate precursor to bone apatitic mineral
Author(s): John-David P. McElderry; Guisheng Zhao; Qian Yang; Gurjit Mandair; Renny T. Franceschi; Michael D. Morris
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Paper Abstract

Bone is a highly specialized connective tissue comprised of cross-linked collagen fibers interspersed with apatitic mineral crystallites of various sizes, shapes, orientation, and composition. However, the nucleation, growth, and propagation of mineral crystallite into the collagenous matrix are not clearly understood. By using a research grade inverted microscope fitted with a line-shaped 830 nm laser and spectrograph, we show that the Raman scatter from mineralizing cell cultures in an incubation chamber can be collected and monitored directly through the bottom of the well-plates over a period of 24 hours. In our studies, murine-derived MC3T3 cells were incubated at 37°C in the presence of 5% CO2 and 85% humidity. Results show a gradual shift in the phosphate ν1 apatitic band center (955-957 cm-1) during the first hour of mineralization. The phosphate ν1 apatitic band width also narrowed during this time. To quantify the amount of crystal growth in vivo, we used a calibration curve derived from X-ray powder diffraction and Raman studies performed on a series of synthetic carbonated apatites and deproteinated mouse femoral specimens. Mineralization in neonatal mouse calvarial culture was observed along the lambdoid suture. Deposition proceeded in a stepwise fashion over the course of ~30 h.

Paper Details

Date Published: 2 March 2010
PDF: 7 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 754849 (2 March 2010); doi: 10.1117/12.842497
Show Author Affiliations
John-David P. McElderry, Univ. of Michigan (United States)
Guisheng Zhao, Univ. of Michigan (United States)
Qian Yang, Univ. of Michigan (United States)
Gurjit Mandair, Univ. of Michigan (United States)
Renny T. Franceschi, Univ. of Michigan (United States)
Michael D. Morris, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong M.D.; Nikiforos Kollias; Henry Hirschberg M.D.; Kenton W. Gregory M.D.; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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