Proceedings Paper
Total internal reflection holographic microscopy for quantitative phase characterization of cellular adhesion| Format | Member Price | Non-Member Price |
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Paper Abstract
A new form of near-field microscopy is presented using digital holography for quantitative phase imagery and
characterization of cell-substrate interfaces. This imaging technique, termed total internal reflection holographic
microscopy (TIRHM), utilizes an evanescent wave phase shift from the presence of cellular organisms, membranes,
adhesions, and tissue structures on a prism face in order to modulate an object beam wavefront in a digital holographic
microscope. Quantitative phase images of live cellular specimens are presented.
Paper Details
Date Published: 11 February 2010
PDF: 12 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756803 (11 February 2010); doi: 10.1117/12.842470
Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)
PDF: 12 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756803 (11 February 2010); doi: 10.1117/12.842470
Show Author Affiliations
William M. Ash III, Univ. of South Florida (United States)
David Clark, Univ. of South Florida (United States)
David Clark, Univ. of South Florida (United States)
Chun-Min Lo, Univ. of South Florida (United States)
Myung K. Kim, Univ. of South Florida (United States)
Myung K. Kim, Univ. of South Florida (United States)
Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)
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