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Proceedings Paper

Total internal reflection holographic microscopy for quantitative phase characterization of cellular adhesion
Author(s): William M. Ash III; David Clark; Chun-Min Lo; Myung K. Kim
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Paper Abstract

A new form of near-field microscopy is presented using digital holography for quantitative phase imagery and characterization of cell-substrate interfaces. This imaging technique, termed total internal reflection holographic microscopy (TIRHM), utilizes an evanescent wave phase shift from the presence of cellular organisms, membranes, adhesions, and tissue structures on a prism face in order to modulate an object beam wavefront in a digital holographic microscope. Quantitative phase images of live cellular specimens are presented.

Paper Details

Date Published: 11 February 2010
PDF: 12 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756803 (11 February 2010); doi: 10.1117/12.842470
Show Author Affiliations
William M. Ash III, Univ. of South Florida (United States)
David Clark, Univ. of South Florida (United States)
Chun-Min Lo, Univ. of South Florida (United States)
Myung K. Kim, Univ. of South Florida (United States)


Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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