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Proceedings Paper

Molecular imaging of neutropilin-1 receptor using photoacoustic spectroscopy in breast tumors
Author(s): Keith M. Stantz; Minsong Cao; Bo Liu; Kathy D. Miller; Lili Guo
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Paper Abstract

Purpose: Our purpose is to develop and test a molecular probe that can detect the expression of neutropilin-1 receptor (NPR-1) in vivo using fluorescence imaging and photoacoustic spectroscopy. Introduction: NPR-1 is expressed on endothelial cells and some breast cancer cells, and binds to vascular endothelial growth factor VEGF165, a growth factor associated with pathological tumor angiogenesis. This receptor is coexpressed with VEGFR2 and shown to enhance the binding of VEGF165; therefore, it has the potential to be used as a marker of angiogenic activity and targeted for therapy. Material and Methods: A peptide specific to NPR-1 receptor was synthesized and conjugated to a NIR fluorochrome (IRDye800CW) and was intravenously injected into mice with breast tumors (MCF7VEGF). Probe kinetics was monitored in vivo via near infrared fluorescence (NIRF) within an optical imager for up to 72 hours within the tumor and compared to other organs (liver, muscle) for binding specificity. A multivariate fitting algorithm was used to spectrally deconvolve the IRDye800CW from endogenous hemoglobin signature (hemoglobin concentration and oxygen saturation). Results: Dynamics of the NIR fluorescence signal within the first hour after injection indicates specific binding compared to muscle, with an average tumor-to-muscle ration of 2.00 (+/- 0.27). Spectral analysis clearly indentified the presence of the NPR-1 probe. Based on calibration data, the average tumor concentration from both NIRF and PCT-S was measured to be ~200-300nM. Conclusion: These preliminary results show the capability of PCT to image an exogenous probe in vivo in addition to its hemoglobin state.

Paper Details

Date Published: 25 February 2010
PDF: 7 pages
Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75641O (25 February 2010); doi: 10.1117/12.842271
Show Author Affiliations
Keith M. Stantz, Purdue Univ. (United States)
Indiana Univ. (United States)
Minsong Cao, Indiana Univ. (United States)
Bo Liu, Purdue Univ. (United States)
Kathy D. Miller, Indiana Univ. (United States)
Lili Guo, Indiana Univ. (United States)

Published in SPIE Proceedings Vol. 7564:
Photons Plus Ultrasound: Imaging and Sensing 2010
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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