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Proceedings Paper

Two photon microscopy intravital study of DC-mediated anti-tumor response of NK cells
Author(s): Michele Caccia; Tatiana Gorletta; Laura Sironi; Ivan Zanoni; Cristina Salvetti; Maddalena Collini; Francesca Granucci; Giuseppe Chirico
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Paper Abstract

Recent studies have demonstrated that dendritic cells (DCs) play a crucial role in the activation of Natural Killer cells (NKs) that are responsible for anti-tumor innate immune responses. The focus of this report is on the role of pathogen associated molecular pattern (PAMP) activated-DCs in inducing NK cell-mediated anti-tumor responses. Mice transplanted sub-cute (s.c.) with AK7 cells, a mesothelioma cell line sensitive to NK cell responses, are injected with fluorescent NK cells and DC activation is then induced by s.c. injection of Lipopolysaccharide (LPS). Using 4 dimensional tracking we follow the kinetic behavior of NK cells at the Draining Lymph-Node (DLN). As control, noninflammatory conditions are also evaluated. Our data suggest that NK cells are recruited to the DLN where they can interact with activated-DCs with a peculiar kinetic behavior: short lived interactions interleaved by rarer longer ones. We also found that the changes in the NK dynamic behavior in inflammatory conditions clearly affect relevant motility parameters such as the instantaneous and average velocity and the effective diffusion coefficient. This observation suggests that NK cells and activated-DCs might efficiently interact in the DLN, where cells could be activated. Therefore the interaction between activated-DCs and NK cells in DLN is not only a reality but it may be also crucial for the start of the immune response of the NKs.

Paper Details

Date Published: 11 February 2010
PDF: 6 pages
Proc. SPIE 7565, Biophotonics and Immune Responses V, 75650Q (11 February 2010); doi: 10.1117/12.842214
Show Author Affiliations
Michele Caccia, Univ. degli Studi di Milano-Bicocca (Italy)
Tatiana Gorletta, Univ. degli Studi di Milano-Bicocca (Italy)
Laura Sironi, Univ. degli Studi di Milano-Bicocca (Italy)
Ivan Zanoni, Univ. degli Studi di Milano-Bicocca (Italy)
Cristina Salvetti, Univ. degli Studi di Milano-Bicocca (Italy)
Maddalena Collini, Univ. degli Studi di Milano-Bicocca (Italy)
Francesca Granucci, Univ. degli Studi di Milano-Bicocca (Italy)
Giuseppe Chirico, Univ. degli Studi di Milano-Bicocca (Italy)

Published in SPIE Proceedings Vol. 7565:
Biophotonics and Immune Responses V
Wei R. Chen, Editor(s)

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