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Proceedings Paper

Low-cost fluorescence microscopy for point-of-care cell imaging
Author(s): Michael J. Lochhead; Jeff Ives; Monique Givens; Marie Delaney; Kevin Moll; Christopher J. Myatt
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Paper Abstract

Fluorescence microscopy has long been a standard tool in laboratory medicine. Implementation of fluorescence microscopy for near-patient diagnostics, however, has been limited due to cost and complexity associated with traditional fluorescence microscopy techniques. There is a particular need for robust, low-cost imaging in high disease burden areas in the developing world, where access to central laboratory facilities and trained staff is limited. Here we describe a point-of-care assay that combines a disposable plastic cartridge with an extremely low cost fluorescence imaging instrument. Based on a novel, multi-mode planar waveguide configuration, the system capitalizes on advances in volume-manufactured consumer electronic components to deliver an imaging system with minimal moving parts and low power requirements. A two-color cell imager is presented, with magnification optimized for enumeration of immunostained human T cells. To demonstrate the system, peripheral blood mononuclear cells were stained with fluorescently labeled anti-human-CD4 and anti-human-CD3 antibodies. Registered images were used to generate fractional CD4+ and CD3+ staining and enumeration results that show excellent correlation with flow cytometry. The cell imager is under development as a very low cost CD4+ T cell counter for HIV disease management in limited resource settings.

Paper Details

Date Published: 25 February 2010
PDF: 6 pages
Proc. SPIE 7572, Optical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics, 75720B (25 February 2010); doi: 10.1117/12.842099
Show Author Affiliations
Michael J. Lochhead, Precision Photonics Corp. (United States)
Jeff Ives, Precision Photonics Corp. (United States)
Monique Givens, Precision Photonics Corp. (United States)
Marie Delaney, Precision Photonics Corp. (United States)
Kevin Moll, Precision Photonics Corp. (United States)
Christopher J. Myatt, Precision Photonics Corp. (United States)

Published in SPIE Proceedings Vol. 7572:
Optical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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