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Proceedings Paper

Spectrally resolved fluorescence lifetime imaging: new developments and applications
Author(s): A. Rueck; F. Dolp; B. v. Einem; C. A. F. v. Arnim; D. Strat
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Paper Abstract

The fluorescence lifetime of different molecular species is calculated from the measured fluorescence intensity decrease following short pulsed laser excitation, by a multi-channel fitting procedure. In a FRET (Förster Resonant Energy Transfer) experiment the time dependent behaviour of the donor profile is assumed in a first view mono-exponential and the acceptor decay profile is solved analytically. A global minimization fitting algorithm has increased information content than a single channel fitting routine. In a normal FRET-FLIM experiment, the efficiency of FRET is calculated only by considering the kinetics of the donor. However, as will be shown, a considerable improvement could be achieved when time-resolved and spectral-resolved techniques are simultaneously incorporated.

Paper Details

Date Published: 26 February 2010
PDF: 8 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75690Y (26 February 2010); doi: 10.1117/12.841782
Show Author Affiliations
A. Rueck, ILM (Germany)
F. Dolp, ILM (Germany)
B. v. Einem, Univ. Ulm (Germany)
C. A. F. v. Arnim, Univ. Ulm (Germany)
D. Strat, ILM (Germany)

Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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