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Proceedings Paper

Confocal fluorescence detection of cell-based assays using a digital micromirror device
Author(s): Jong-ryul Choi; Jong Hwan Sung; Michael L. Shuler; Donghyun Kim
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Paper Abstract

We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.

Paper Details

Date Published: 10 February 2010
PDF: 5 pages
Proc. SPIE 7596, Emerging Digital Micromirror Device Based Systems and Applications II, 759608 (10 February 2010); doi: 10.1117/12.841738
Show Author Affiliations
Jong-ryul Choi, Yonsei Univ. (Korea, Republic of)
Jong Hwan Sung, Cornell Univ. (United States)
Michael L. Shuler, Cornell Univ. (United States)
Donghyun Kim, Yonsei Univ. (Korea, Republic of)

Published in SPIE Proceedings Vol. 7596:
Emerging Digital Micromirror Device Based Systems and Applications II
Michael R. Douglass; Larry J. Hornbeck, Editor(s)

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