We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (φ = 10 μm) as an equivalent to a cell.

Date Published: 10 February 2010
PDF: 5 pages
Proc. SPIE 7596, Emerging Digital Micromirror Device Based Systems and Applications II, 759608 (10 February 2010); doi: 10.1117/12.841738

Published in SPIE Proceedings Vol. 7596:
Emerging Digital Micromirror Device Based Systems and Applications II
Michael R. Douglass; Larry J. Hornbeck, Editor(s)