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Proceedings Paper

Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles
Author(s): Dayong Jin; James Piper; Jingli Yuan; Robert Leif
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Paper Abstract

In advanced cytometry, a fundamental challenge for rapid specific detection of rare-event micro-organisms is the autofluorescence noise from the complex biological samples. Time-gated luminescence can effectively discriminate labeled cells from autofluorescence background. Recently, a real-time true-colour time-gated luminescence microscopy system has been developed based on the synchronization of a solid-state excitation source and a super-fast optical shutter. We also developed a variety of ultra-bright silica nano-biolabels with multiple luminescence colours and controllable lifetimes in microsecond range. These developments allowed the development of an advanced cell analysis system for real-time background-free imaging and rare-event counting of microsecond-lifetime multi-colour labelled water-borne pathogens.

Paper Details

Date Published: 24 February 2010
PDF: 12 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756819 (24 February 2010); doi: 10.1117/12.841569
Show Author Affiliations
Dayong Jin, Macquarie Univ. (Australia)
James Piper, Macquarie Univ. (Australia)
Jingli Yuan, Dalian Univ. of Technology (China)
Robert Leif, Newport Instruments (United States)

Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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