
Proceedings Paper
Frequency modulation coherent anti-Stokes Raman scattering (FM-CARS) microscopy based on spectral focusing of chirped laser pulsesFormat | Member Price | Non-Member Price |
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Paper Abstract
We demonstrate a new CARS microscopy method based on fast switching of effective vibrational excitation frequency
from chirped femtosecond laser pulses. Broadband pump and Stokes pulses excite a single vibrational mode with a high
spectral resolution when the two pulses are identically chirped and their pulse durations are approaching the dephasing
time of the excited vibrational state. This "spectral focusing" mechanism is applied to CARS microscopy with a single
broadband Ti:Sapphire laser. The vibrational excitation frequency is controlled simply by the time delay between the
pump and Stokes pulses and fast switching of the excitation frequency (~100 kHz) is achieved with a Pockels cell and
polarization optics. Lock-in detection of the difference between the two CARS signals at nearby vibrational frequencies
not only eliminates the non-resonant background but also generates a spectral line shape similar to the spontaneous
Raman scattering. We demonstrate both micro-spectroscopy and vibrational imaging with various samples.
Paper Details
Date Published: 26 February 2010
PDF: 7 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756909 (26 February 2010); doi: 10.1117/12.841497
Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)
PDF: 7 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756909 (26 February 2010); doi: 10.1117/12.841497
Show Author Affiliations
Bi-Chang Chen, The Univ. of Texas at Austin (United States)
Jiha Sung, The Univ. of Texas at Austin (United States)
Jiha Sung, The Univ. of Texas at Austin (United States)
Sang-Hyun Lim, The Univ. of Texas at Austin (United States)
Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)
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