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Proceedings Paper

High-throughput multispot single-molecule spectroscopy
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Paper Abstract

Solution-based single-molecule spectroscopy and fluorescence correlation spectroscopy (FCS) are powerful techniques to access a variety of molecular properties such as size, brightness, conformation, and binding constants. However, this is limited to low concentrations, which results in long acquisition times in order to achieve good statistical accuracy. Data can be acquired more quickly by using parallelization. We present a new approach using a multispot excitation and detection geometry made possible by the combination of three powerful new technologies: (i) a liquid crystal spatial light modulator to produce multiple diffraction-limited excitation spots; (ii) a multipixel detector array matching the excitation pattern and (iii) a low-cost reconfigurable multichannel counting board. We demonstrate the capabilities of this technique by reporting FCS measurements of various calibrated samples as well as single-molecule burst measurements.

Paper Details

Date Published: 25 February 2010
PDF: 11 pages
Proc. SPIE 7571, Single Molecule Spectroscopy and Imaging III, 75710G (25 February 2010); doi: 10.1117/12.841398
Show Author Affiliations
Ryan A. Colyer, Univ. of California, Los Angeles (United States)
Giuseppe Scalia, Univ. of California, Los Angeles (United States)
Taiho Kim, Nesher Technologies (United States)
Ivan Rech, Politecnico di Milano (Italy)
Daniele Resnati, Politecnico di Milano (Italy)
Stefano Marangoni, Politecnico di Milano (Italy)
Massimo Ghioni, Politecnico di Milano (Italy)
Sergio Cova, Politecnico di Milano (Italy)
Shimon Weiss, Univ. of California, Los Angeles (United States)
Xavier Michalet, Univ. of California, Los Angeles (United States)

Published in SPIE Proceedings Vol. 7571:
Single Molecule Spectroscopy and Imaging III
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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