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Proceedings Paper

High performance multimodal CARS microscopy using a single femtosecond source
Author(s): Adrian F. Pegoraro; Aaron D. Slepkov; Andrew Ridsdale; Rodney K. Lyn; Douglas J. Moffatt; John Paul Pezacki; Brian K. Thomas; Libin Fu; Liang Dong; Martin E. Fermann; Albert Stolow
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Paper Abstract

Traditional CARS microscopy using picosecond (ps) lasers has been applied to a wide variety of applications; however, the lasers required are expensive and require an environmentally stable lab. In our work, we demonstrate CARS microscopy using a single femtosecond (fs) laser combined with a photonic crystal fiber (PCF) and optimal chirping to achieve similar performance to the ps case with important added advantages: fs-CARS utilizes versatile source that allows CARS to be combined with other multiphoton techniques (e.g. SHG, TPF) for multimodal imaging without changing laser sources. This provides an attractive entry point for many researchers to the field. Furthermore, optimal chirping in fs-CARS also opens the door to the combination and extension of other techniques used in ps CARS microscopy such as multiplex and FM imaging. The key advantage with chirped fs pulses is that time delay corresponds to spectral scanning and allows for rapid modulation of the resonant CARS signal. The combination of a fs oscillator with a PCF leads to a natural extension of the technology towards an all-fiber source for multimodal multiphoton microscopy. An all-fiber system should be more robust against environmental fluctuations while being more compact than free-space systems. We have constructed and demonstrated a proof of concept all-fiber based source that can be used for simultaneous CARS, TPF and SHG imaging. This system is capable of imaging tissue samples and live cell cultures with 4 μs/pixel dwell time at low average powers.

Paper Details

Date Published: 26 February 2010
PDF: 9 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756908 (26 February 2010); doi: 10.1117/12.841396
Show Author Affiliations
Adrian F. Pegoraro, National Research Council Canada (Canada)
Queen's Univ. (Canada)
Aaron D. Slepkov, National Research Council Canada (Canada)
Andrew Ridsdale, National Research Council Canada (Canada)
Rodney K. Lyn, National Research Council Canada (Canada)
Douglas J. Moffatt, National Research Council Canada (Canada)
John Paul Pezacki, National Research Council Canada (Canada)
Brian K. Thomas, IMRA America, Inc. (United States)
Libin Fu, IMRA America, Inc. (United States)
Liang Dong, IMRA America, Inc. (United States)
Martin E. Fermann, IMRA America, Inc. (United States)
Albert Stolow, National Research Council Canada (Canada)
Queen's Univ. (Canada)

Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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