
Proceedings Paper
Regulatory assembly of the vacuolar proton pump VoV1-ATPase in yeast cells by FLIM-FRETFormat | Member Price | Non-Member Price |
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Paper Abstract
We investigate the reversible disassembly of VOV1-ATPase in life yeast cells by time resolved confocal FRET
imaging. VOV1-ATPase in the vacuolar membrane pumps protons from the cytosol into the vacuole. VOV1-ATPase is
a rotary biological nanomotor driven by ATP hydrolysis. The emerging proton gradient is used for secondary
transport processes as well as for pH and Ca2+ homoeostasis in the cell. The activity of the VOV1-ATPase is regulated
through assembly / disassembly processes. During starvation the two parts of VOV1-ATPase start to disassemble.
This process is reversed after addition of glucose. The exact mechanisms are unknown. To follow the disassembly /
reassembly in vivo we tagged two subunits C and E with different fluorescent proteins. Cellular distributions of C
and E were monitored using a duty cycle-optimized alternating laser excitation scheme (DCO-ALEX) for time
resolved confocal FRET-FLIM measurements.
Paper Details
Date Published: 26 February 2010
PDF: 8 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75690W (26 February 2010); doi: 10.1117/12.841169
Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)
PDF: 8 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75690W (26 February 2010); doi: 10.1117/12.841169
Show Author Affiliations
Stefan Ernst, Univ. Stuttgart (Germany)
Claire Batisse, EMBL Heidelberg (Germany)
Nawid Zarrabi, Univ. Stuttgart (Germany)
Claire Batisse, EMBL Heidelberg (Germany)
Nawid Zarrabi, Univ. Stuttgart (Germany)
Bettina Böttcher, EMBL Heidelberg (Germany)
Univ. of Edinburgh (United Kingdom)
Michael Börsch, Univ. Stuttgart (Germany)
Univ. of Edinburgh (United Kingdom)
Michael Börsch, Univ. Stuttgart (Germany)
Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)
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