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Proceedings Paper

High-speed CARS spectral imaging using acousto optic tunable filter
Author(s): Mamoru Hashimoto; Takeo Minamikawa; Tsutomu Araki
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Paper Abstract

We developed a high speed CARS (coherent anti-Stokes Raman scattering) spectral-imaging system using an acousto optic tunable filter and multi-focus excitation system. We compared two methods of CARS emission filtering and CARS excitation filtering. In both case, two laser pulses with narrow band (picosecond laser) and broad band (femotosecond laser)were used for the light source. For CARS emission filtering, the generated CARS was filtered by an AOTF, and for excitation filtering the broad band femtosecond laser pulse were filtered by an AOTF before excitation. The experimental results indicated that the CARS emission filtering was suitable for CARS microscopy.

Paper Details

Date Published: 26 February 2010
PDF: 7 pages
Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75690Q (26 February 2010); doi: 10.1117/12.841078
Show Author Affiliations
Mamoru Hashimoto, Osaka Univ. (Japan)
Takeo Minamikawa, Osaka Univ. (Japan)
Tsutomu Araki, Osaka Univ. (Japan)


Published in SPIE Proceedings Vol. 7569:
Multiphoton Microscopy in the Biomedical Sciences X
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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