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Proceedings Paper

Four-dimensional multi-site two-photon excitation
Author(s): Vincent R. Daria; Christian Stricker; Richard Bowman; Hans-A. Bachor; Stephen Redman
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Paper Abstract

We use the holographic method to project an arbitrary array of diffraction-limited focal spots suitable for multi-site twophoton excitation. The spot array can be projected arbitrarily within a three-dimensional (3D) volume, while the fourth dimension in time is attributed to high temporal resolution via high-speed non-iterative calculation of the hologram using a video graphics accelerator board. We show that the spots have sufficient energy and spatiotemporal photon density for localized two-photon excitation at individual spots in the array. The significance of this work points to 3D microscopy, non-linear micro-fabrication, volume holographic optical storage and biomedical instrumentation. In neuroscience, timecritical release of neurotransmitters at multiple sites within complex dendritic trees of neurons can lead to insights on the mechanisms of information processing in the brain.

Paper Details

Date Published: 2 March 2010
PDF: 8 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75483T (2 March 2010); doi: 10.1117/12.840782
Show Author Affiliations
Vincent R. Daria, The Australian National Univ. (Australia)
John Curtin School of Medical Research, Australian National Univ. (Australia)
Christian Stricker, John Curtin School of Medical Research, Australian National Univ. (Australia)
Richard Bowman, SUPA, Univ. of Glasgow (United Kingdom)
Hans-A. Bachor, The Australian National Univ. (Australia)
Stephen Redman, John Curtin School of Medical Research, Australian National Univ. (Australia)

Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong M.D.; Nikiforos Kollias; Henry Hirschberg M.D.; Kenton W. Gregory M.D.; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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