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Proceedings Paper

In situ cell cycle phase determination using Raman spectroscopy
Author(s): Yusuke Oshima; Tatsuji Takenaka; Hidetoshi Sato; Chie Furihata
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Paper Abstract

Raman spectroscopy is a powerful tool for analysis of the chemical composition in living tissue and cells without destructive processes such as fixation, immunostaining, and fluorescence labeling. Raman microspectroscopic technique enables us to obtain a high quality spectrum from a single living cell. We demonstrated in situ cell cycle analysis with Raman microspectroscopy with the excitation wavelength of 532 nm. Cell cycle phases, G0/G1 and G2/M were able to be identified in the present study. The result of in situ Raman analysis was evaluated with flow cytometry analysis. Although the Raman spectra of living cells showed complex patterns during cell cycle, several Raman bands could be useful as markers for the cell cycle identification. A single cell analysis using Raman microspectroscopy predicted a possibility to observe directly molecular dynamics intracellular molecules of proteins, lipids and nucleic acids. Our current study focused on cytoplasm region and resonant Raman signals of cytochrome c in mitochondrion, and discussed how the Raman signals from cellular components contribute to the Raman spectral changes in cell cycle change in the human living cell (lung cancer cell).

Paper Details

Date Published: 11 February 2010
PDF: 6 pages
Proc. SPIE 7560, Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry, 756008 (11 February 2010); doi: 10.1117/12.840593
Show Author Affiliations
Yusuke Oshima, Aoyama Gakuin Univ. (Japan)
RIKEN (Japan)
Tatsuji Takenaka, Aoyama Gakuin Univ. (Japan)
Hidetoshi Sato, RIKEN (Japan)
Kwansei Gakuin Univ. (Japan)
Chie Furihata, Aoyama Gakuin Univ. (Japan)

Published in SPIE Proceedings Vol. 7560:
Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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