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Proceedings Paper

Flow visualization and flow cytometry with holographic video microscopy
Author(s): Fook Chiong Cheong; Bo Sun; Remi Dreyfus; Jesse Amato-Grill; Ke Xiao; Lisa Dixon; David G. Grier
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Paper Abstract

The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution, and simultaneously to measure their sizes and refractive indexes. An efficient particle-tracking algorithm automates initial position estimation with sufficient accuracy to enable unattended holographic particle tracking and characterization. In this work, we demonstrated this approach to flow visualization in a microfluidic channel and also to flow cytometry of micrometer-scale colloidal spheres.

Paper Details

Date Published: 10 February 2010
PDF: 6 pages
Proc. SPIE 7619, Practical Holography XXIV: Materials and Applications, 761905 (10 February 2010); doi: 10.1117/12.840550
Show Author Affiliations
Fook Chiong Cheong, New York Univ. (United States)
Bo Sun, New York Univ. (United States)
Remi Dreyfus, New York Univ. (United States)
Jesse Amato-Grill, New York Univ. (United States)
Ke Xiao, New York Univ. (United States)
Lisa Dixon, New York Univ. (United States)
David G. Grier, New York Univ. (United States)

Published in SPIE Proceedings Vol. 7619:
Practical Holography XXIV: Materials and Applications
Hans I. Bjelkhagen; Raymond K. Kostuk, Editor(s)

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