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Proceedings Paper

Assessment of laser photobiomodulation and polarized light on the healing of cutaneous wounds on euthyroid and hypothyroid induced rats
Author(s): Luciana Maria Pedreira Ramalho; Barbara Mayoral Pedroso Weyll; Maíra Dória M. da Costa Lino; Maria Jose Pedreira Ramalho; Antonio Luis Barbosa Pinheiro
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Paper Abstract

The aim of this study was to assess the influence of low-level laser therapy (LLLT) or polarized light (PL) in cutaneous wound healing of hypothyroid rats at dosages of 20 or 40J/cm2. Bioestimulatory effects of Laser radiation and Polarized light are recognized alternative therapies to improve healing on systemic disease patients, but their usefulness in the improvement of hypothyroidism healing impairment is uncertain till date. Forty Wistar rats were used in this study. Hypothyroidism was propylthiouracil- induced. Standard excisional cutaneous wounds were created without suturing and LLLT (λ660nm, 30mW, φ 3mm) or PL (λ 400-2000nm, 40mW, φ 10mm) was applied every 48 hours up to seven days on experimental groups. The rats were killed on the eighth day when wound contraction was assessed. The healing features were evaluated by light microscopy (H/E and Sirius Red). The cutaneous wounds of hypothyroid rats showed delayed healing process characterized by reduced thickness of epithelial layers, incipient formation of disorganized collagen fibers and wound contraction to a lesser extent (FISHER, p=0.0276), when compared to the euthyroid group. The use of both the Laser and Polarized Light on hypothyroid rats increased the amount of fibroblasts and the thickness of collagen fibers, especially on the L 20J/cm2 group. Euthyroid rats have still demonstrated more regular collagen fibers pattern than hypothyroid rats. It was therefore concluded that hypothyroidism delays wound healing and both Laser photobiomodulation and Polarized Light at 20j/cm2 dosages had improved the healing process in hypothyroid rats.

Paper Details

Date Published: 25 February 2010
PDF: 6 pages
Proc. SPIE 7552, Mechanisms for Low-Light Therapy V, 75520P (25 February 2010); doi: 10.1117/12.840508
Show Author Affiliations
Luciana Maria Pedreira Ramalho, Univ. Federal da Bahia (Brazil)
Barbara Mayoral Pedroso Weyll, Univ. Federal da Bahia (Brazil)
Maíra Dória M. da Costa Lino, Univ. Federal da Bahia (Brazil)
Maria Jose Pedreira Ramalho, Univ. Federal da Bahia (Brazil)
Antonio Luis Barbosa Pinheiro, Univ. Federal da Bahia (Brazil)

Published in SPIE Proceedings Vol. 7552:
Mechanisms for Low-Light Therapy V
Michael R. Hamblin; Ronald W. Waynant; Juanita Anders, Editor(s)

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