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Proceedings Paper

Plasticity of climbing fibers after laser axotomy
Author(s): A. L. Allegra Mascaro; P. Cesare; L. Sacconi; G. Grasselli; P. Strata; F. S. Pavone
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Paper Abstract

In the adult nervous system, different population of neurons corresponds to different regenerative behavior. Although previous works show that olivocerebellar fibers are capable of axonal regeneration in a suitable environment as a response to injury1, we have hitherto no details about the real dynamics of fiber regeneration. We coupled two photon imaging to laser-induced lesions to perform in vivo multiphoton nanosurgery in the CNS of living mice expressing fluorescent proteins to investigate the reparative properties of Climbing Fibers (CFs) in the adult CNS, following the time evolution of this plastic process in vivo. Here we show that a regenerative event may take place in a murine model in the days that follow a sub-micrometric lesion on the distal portion of the climbing fiber. Furthermore this unique model could allow, through manipulation of the viral vector, to explore in detail the biochemical mechanisms underlying the reparative process. The great potential of long-term two photon imaging, coupled to genetic manipulation, opens great opportunities to further investigate the dynamic properties of neurons and their rearrangement following an injury.

Paper Details

Date Published: 2 March 2010
PDF: 4 pages
Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 754853 (2 March 2010);
Show Author Affiliations
A. L. Allegra Mascaro, European Lab. for Non-Linear Spectroscopy, Univ. of Florence (Italy)
P. Cesare, EBRI, Santa Lucia Foundation (Italy)
Univ. of Turin (Italy)
L. Sacconi, European Lab. for Non-Linear Spectroscopy, Univ. of Florence (Italy)
G. Grasselli, EBRI, Santa Lucia Foundation (Italy)
P. Strata, EBRI, Santa Lucia Foundation (Italy)
Univ. of Turin (Italy)
F. S. Pavone, European Lab. for Non-Linear Spectroscopy, Univ. of Florence (Italy)

Published in SPIE Proceedings Vol. 7548:
Photonic Therapeutics and Diagnostics VI
Anita Mahadevan-Jansen; Andreas Mandelis; Brian Jet-Fei Wong M.D.; Nikiforos Kollias; Henry Hirschberg M.D.; Kenton W. Gregory M.D.; Reza S. Malek; E. Duco Jansen; Guillermo J. Tearney; Steen J. Madsen; Bernard Choi; Justus F. R. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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