
Proceedings Paper
Ultrasensitive label-free detection and quantitation of DNA hybridization via terahertz spectrometryFormat | Member Price | Non-Member Price |
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Paper Abstract
Time-domain terahertz (THz) spectrometry has been used to analyze DNA hybridization state and its quantitation in
a label-free manner. Time-resolved THz signal (or temporal signal) converted to frequency domain constitutes a
signature of a given molecular "event" (e.g., a vibrational or a conformational state). The temporal signal provides a
means of probing a molecular event in an appropriate time window. This is a unique ability of this technique
because different molecular events exhibit different time response based on their physical and chemical nature.
Conformational difference of a given molecule results in different signature with an appropriate time response that
can be accurately probed by a terahertz temporal signal. In this work we discriminate between single stranded and
double stranded 25-mer oligonucleotides via spectral signature. For each species, different peaks were identified;
however, the peaks are distinctly different allowing an easy comparison. Additionally, temporal transmission spectra
of the DNA specimens were collected at normal temperature and atmosphere. The peak value extracted from the
temporal spectra exhibit a power law behavior over a region spanning from 13.6 femto-molar to 0.136 nano-molar.
The results clearly demonstrate the ability of the spectrometer to detect a minute amount of biomolecules in a labelfree
fashion. This capability can be used as a diagnostic tool, as well as for studying molecular reactions such as
mutation.
Paper Details
Date Published: 24 February 2010
PDF: 7 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756810 (24 February 2010); doi: 10.1117/12.839542
Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)
PDF: 7 pages
Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756810 (24 February 2010); doi: 10.1117/12.839542
Show Author Affiliations
Anis Rahman, Applied Research and Photonics Inc. (United States)
Bruce Stanley, The Pennsylvania State Univ. College of Medicine (United States)
Bruce Stanley, The Pennsylvania State Univ. College of Medicine (United States)
Aunik K. Rahman, Applied Research and Photonics Inc. (United States)
Published in SPIE Proceedings Vol. 7568:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)
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