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Proceedings Paper

Mechanical behavior study of single cell contraction by digital image correlation technique
Author(s): Jianyong Huang; Jia Wu; Lei Qin; Tao Zhu; Chunyang Xiong; Youyi Zhang; Jing Fang
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Paper Abstract

Precise determination of cellular traction forces has important significance in assessing cellular mechanical characteristic on micro/nano scale. Elastic substrate method is a useful way to study cellular traction forces, in which the cells are cultured on elastic gel substrate marked by randomly embedding fluorescent microbeads and they exert mechanical forces on the film to cause deformation of the substrate material. In this paper, we investigate the acquisition of deformation field induced by single cardiac myocyte by using digital image correlation (DIC) technique. In view of the fact that cellular force restoration is essentially an ill-posed inverse problem, which implies that the force reconstruction is susceptible to the input displacement noise, we develop a novel optimal filtering scheme in two-dimensional Fourier space to restrain displacement noise amplification. Experiments of traction force recovery for a real cardiac myocyte indicate the optimal scheme in combination with the DIC method enables us to reconstruct cellular traction fields with high accuracy.

Paper Details

Date Published: 25 August 2009
PDF: 6 pages
Proc. SPIE 7375, ICEM 2008: International Conference on Experimental Mechanics 2008, 73754X (25 August 2009); doi: 10.1117/12.839324
Show Author Affiliations
Jianyong Huang, Peking Univ. (China)
Jia Wu, Peking Univ. (China)
Lei Qin, Peking Univ. (China)
Peking Univ. Third Hospital (China)
Tao Zhu, Peking Univ. (China)
Chunyang Xiong, Peking Univ. (China)
Youyi Zhang, Peking Univ. (China)
Peking Univ. Third Hospital (China)
Jing Fang, Peking Univ. (China)

Published in SPIE Proceedings Vol. 7375:
ICEM 2008: International Conference on Experimental Mechanics 2008
Xiaoyuan He; Huimin Xie; YiLan Kang, Editor(s)

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