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Proceedings Paper

Phase reconstruction of living human embryonic kidney 293 cells based on two off-axis holograms
Author(s): Wenjing Zhou; Yingjie Yu; Yanhong Duan; Anand Asundi
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Paper Abstract

In this paper, we validate experimentally the potential of off-axis digital micro-holography for 3D image reconstruction of a live Human Embryonic Kidney 293(HEK293) cell that is widely used as transfection and expression. A subtraction method of two off-axis holograms to reconstruct the phase of the live microscopic object is discussed. The presented subtraction method can remove some main noise, for example, the quadratic phase aberration introduced by microscope objective (MO), other phase aberration introduced by the liquid in tank and other interference noise introduced by the optical parts. Thus an improvement in the measurement precision of live cells in aqueous solution is observed. The potential of this method is demonstrated by providing the phase reconstruction results of a phase grating and a single HEK293 cell. The results showed good correspondence to the actual character of HEK293 cell prove the capability of digital micro-holography as a tool to monitor the dynamic transfection process of the living HEK 293 cells.

Paper Details

Date Published: 24 August 2009
PDF: 8 pages
Proc. SPIE 7375, ICEM 2008: International Conference on Experimental Mechanics 2008, 737502 (24 August 2009); doi: 10.1117/12.838970
Show Author Affiliations
Wenjing Zhou, Shanghai Univ. (China)
Yingjie Yu, Shanghai Univ. (China)
Yanhong Duan, Shanghai Univ. (China)
Anand Asundi, Nanyang Technological Univ. (Singapore)

Published in SPIE Proceedings Vol. 7375:
ICEM 2008: International Conference on Experimental Mechanics 2008
Xiaoyuan He; Huimin Xie; YiLan Kang, Editor(s)

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